Recombinant Human HSD11B1 Protein, His-tagged

• Cat.No.: HSD11B1-13951H
• Product Overview: Recombinant Human HSD11B1 Protein (1-292 aa) is produced by E. coli-derived expression system. This protein is fused with a His tag at the N-terminal.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58 mM Na2HPO4,17 mM NaH2PO4, 68 mM NaCl, pH7.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
• Molecular Mass: 32 kDa
• Protein length: 1-292 aa
• AA Sequence: MAFMKKYLLPILGLFMAYYYYSANE EFRPEMLQGKKVIVTGASKGIGREM AYHLAKMGAHVVVTARSKETLQKVV SHCLELGAASAHYIAGTMEDMTFAE QFVAQAGKLMGGLDMLILNHITNTS LNLFHDDIHHVRKSMEVNFLSYVVL TVAALPMLKQSNGSIVVVSSLAGKV AYPMVAAYSASKFALDGFFSSIRKE YSVSRVNVSITLCVLGLIDTETAMK AVSGIVHMQAAPKEECALEIIKGGA LRQEEVYYDSSLWTTLLIRNPCRKI LEFLYSTSYNMDRFINK
• Purity: > 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Stability: Store for up to 12 months at -20 centigrade to -80 centigrade as lyophilized powder.
• Storage: Short-term storage: Store at 2-8 centigrade for (1-2 weeks).
  Long-term storage: Aliquot and store at -20 centigrade to -80 centigrade for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
  Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: HSD11B1 hydroxysteroid (11-beta) dehydrogenase 1 [ Homo sapiens ]
• Official Symbol: HSD11B1
• Synonyms: HSD11B1; SDR26C1; HDL; 11-DH; HSD11; HSD11B; HSD11L; 11-beta-HSD1; MGC13539
• Gene ID: 3290
• mRNA Refseq: NM_001206741
• Protein Refseq: NP_001193670
• MIM: 600713