Recombinant Human POLD4, His-tagged

• Cat.No.: POLD4-2282H
• Product Overview: Recombinant human POLD4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: POLD4 is the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3" to 5" exonuclease activity and plays a critical role in DNA replication and repair. The protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT
• Molecular Mass: 14.8 kDa
• Protein length: 130aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGRKRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml

Gene Information

• Gene Name: POLD4 polymerase (DNA-directed), delta 4 [ Homo sapiens ]
• Official Symbol: POLD4
• Synonyms: POLD4; polymerase (DNA-directed), delta 4; DNA polymerase delta subunit 4; DNA polymerase delta smallest subunit p12; p12; POLDS
• Gene ID: 57804
• mRNA Refseq: NM_001256870
• Protein Refseq: NP_001243799
• MIM: 611525
• UniProt ID: Q9HCU8
• Chromosome Location: 11q13
• Pathway: Base Excision Repair, organism-specific biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; DNA Repair, organism-specific biosystem
• Function: DNA-directed DNA polymerase activity; nucleotidyltransferase activity; protein binding; transferase activity