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Active Recombinant Mouse Il4

Cat.No. : Il4-297M
Product Overview : Recombinant Mouse Il4 was expressed in CHO Cells.
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Description : Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet.
Source : CHO Cells
Species : Mouse
Form : Lyophilized after extensive dialysis against PBS.
Bio-activity : ED50< 2 ng/ml, measured in a cell proliferation assay using murine ht-2 cells, corresponding to a specific activity of>5 x 105 units/mg.
Molecular Mass : 15 kDa, observed by reducing SDS-PAGE.
AA Sequence : His23-Ser140.
Endotoxin : < 0.2 eu/μg, determined by lal>
Purity : >95% as analyzed by SDS-PAGE and HPLC.
Storage : Lyophilized recombinant protein remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmIL-4 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Reconstitution : Reconstituted in ddH2O or PBS at 100 μg/ml.
Publication :
LIF regulates CXCL9 in tumor-associated macrophages and prevents CD8+ T cell tumor-infiltration impairing anti-PD1 therapy (2019)
Alterations in the development of a M2 phenotype in glial cells: rotenone in vitro model of Parkinson's disease (2017)
Altered expression of the immunoregulatory ligand-receptor pair CD200-CD200R1 in the brain of Parkinson’s disease patients (2022)
Parkinsonian neurotoxicants impair the anti-inflammatory response induced by IL4 in glial cells: involvement of the CD200-CD200R1 ligand-receptor pair (2020)
Gene Name : Il4 interleukin 4 [ Mus musculus ]
Official Symbol : Il4
Synonyms : IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1;
Gene ID : 16189
mRNA Refseq : NM_021283
Protein Refseq : NP_067258
UniProt ID : P07750
Pathway : Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem;
Function : cytokine activity; cytokine receptor binding; growth factor activity; interleukin-4 receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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10/17/2021

    By testing immunogenicity in animal models, detect whether an immune response is induced.

    11/09/2020

      The structure of the recombinant protein was analyzed by nuclear magnetic resonance (NMR), X-ray crystallography or electron microscopy, and the results showed that the protein was stable.

      05/06/2020

        This protein has good stability, and the test results of heat resistance, acid and alkali resistance and protease degradation resistance are good.

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