"IDE" Related Products


Recombinant Human IDE, His-tagged

Cat.No.: IDE-267H
Product Overview: Recombinant Human IDE (Met42-Leu1019) fused with an N-terminal Met and 7-His tag, was expressed in Sf 21 (baculovirus).
Description: This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein''s function are associated with Alzheimer''s disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.
Source: Sf 21 (baculovirus)
Species: Human
Tag: His
Predicted N Terminal: Met
Formulation: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Brij-35 and Glycerol.
Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is >1,000 pmol/min/µg.
Molecular Mass: 114 kDa
Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage: Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
Gene Name: IDE insulin-degrading enzyme [ Homo sapiens (human) ]
Official Symbol: IDE
Synonyms: IDE; INSULYSIN; insulin-degrading enzyme; insulinase; insulin protease; Abeta-degrading protease; EC
Gene ID: 3416
mRNA Refseq: NM_004969
Protein Refseq: NP_004960
MIM: 146680
UniProt ID: P14735
Chromosome Location: 10q23-q25
Pathway: Alzheimer''s disease
Function: ATP binding; beta-amyloid binding; glycoprotein binding

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