Recombinant human TRIAP1, His-tagged

• Cat.No.: TRIAP1-2431H
• Product Overview: Recombinant human TRIAP1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: TP53-regulated inhibitor of apoptosis 1, also known as TRIAP1, contains a p53-binding site within its second exon and the reduction of expression by small interfering RNA enhanced apoptosis, whereas overexpression protected cells from apoptosis caused by DNA damage. TRIAP1 is induced significantly when cells have a low level of genotoxic stresses, but not when DNA damage is severe. TRIAP1 can modulate apoptotic pathways through interaction with Hsp70 that probably inhibits activity of apoptosis protease activating factor-1.
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 11.2kDa (99aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNSVGEA CTDMKREYDQ CFNRWFAEKF LKGDSSGDPC TDLFKRYQQC VQKAIKEKEI PIEGLEFMGHGKEKPENSS
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TRIAP1?TP53 regulated inhibitor of apoptosis 1 [?Homo sapiens(human) ]
• Official Symbol: TRIAP1
• Synonyms: TRIAP1; WF-1; MDM35; P53CSV; HSPC132; TP53 regulated inhibitor of apoptosis 1; protein 15E1.1; p53-inducible cell-survival factor; mitochondrial distribution and morphology 35 homolog
• Gene ID: 51499
• mRNA Refseq: NM_016399
• Protein Refseq: NP_057483
• MIM: 614943
• UniProt ID: O43715
• Chromosome Location: 12q24.31
• Pathway: Direct p53 effectors
• Function: cysteine-type endopeptidase inhibitor activity involved in apoptotic process