Active Recombinant Human NMNAT1, His-tagged

• Cat.No.: NMNAT1-25H
• Product Overview: Recombinant Human NMNAT1 (Met1-Thr279) fused with 6­His tag at C­terminal, was expressed in Sf 21 (baculovirus).

Specification

• Description: This gene encodes an enzyme which catalyzes a key step in the biosynthesis of the coenzyme NAD. The encoded protein is one of several nicotinamide nucleotide adenylyltransferases. Studies in Drosophila and mammalian neurons have shown the encoded protein can confer protection to damaged neurons. This protection requires enzymatic activity which increases NAD levels and activates a nuclear deacetylase which is the protective molecule. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14 and 15.
• Source: Sf 21 (baculovirus)
• Species: Human
• Tag: His
• Predicted N Terminal: Ser4
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl, Glycerol and DTT.
• Bio-activity: Measured by the production of NAD+, which is converted to NADH by alcohol dehydrogenase. The specific activity is >2,500 pmol/min/µg.
• Molecular Mass: 32 kDa
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: NMNAT1 nicotinamide nucleotide adenylyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: NMNAT1
• Synonyms: NMNAT1; LCA9; NMNAT; PNAT1; nicotinamide nucleotide adenylyltransferase 1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1; pyridine nucleotide adenylyltransferase 1; nicotinate-nucleotide adenylyltransferase 1; EC 2.7.7.1; EC 2.7.7.18
• Gene ID: 64802
• mRNA Refseq: NM_022787
• Protein Refseq: NP_073624
• MIM: 608700
• UniProt ID: Q9HAN9
• Chromosome Location: 1p36.22
• Pathway: Metabolism; Metabolism of vitamins and cofactors; Metabolism of water-soluble vitamins and cofactors
• Function: ATP binding; nicotinamide-nucleotide adenylyltransferase activity; nicotinamide-nucleotide adenylyltransferase activity