| Description : |
Streptavidin is a 60 kDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI).Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting. |
| Source : |
E. coli |
| Species : |
Streptomyces avidinii |
| Tag : |
N/A |
| Predicted N Terminal : |
Met |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization. |
| Bio-activity : |
One unit is defined as the amount of Streptavidin required to bind 1.0 μg biotin by HABA-Avidin method.14-15 units per mg protein supplied at 1 mg/ml. |
| Molecular Mass : |
15.4 kDa. DTT-reduced Protein migrates as 15 kDa. |
| Endotoxin : |
Less than 1.0 EU per μg of the Streptavidin by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC). |
