Recombinant Human SPHK1, Gly & Pro tagged

• Cat.No.: SPHK1-263H
• Product Overview: Recombinant Human SPHK1 (Q9NYA1-1) (Met1-Leu384), fused with two additional amino acids (Gly & Pro) at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Source: Baculovirus-Insect cells
• Species: Human
• Tag: Gly & Pro
• Predicted N Terminal: Gly
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human SPHK1 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The recombinant protein migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens ]
• Official Symbol: SPHK1
• Gene ID: 8877
• mRNA Refseq: NM_001142601
• Protein Refseq: NP_001136073
• MIM: 603730
• UniProt ID: Q9NYA1
• Chromosome Location: 17q25.2