Active Recombinant Rat MET protein(Met1-Asn932), hFc-tagged

• Cat.No.: MET-382R
• Product Overview: Recombinant Rat MET (Q2IBC7) (Met1-Asn932) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-hFc
• Protein length: Met1-Asn932
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Rat HGF at 2 μg/ml (100 μl/well) can bind Rat c-MET hFc, the EC50 of Rat c-MET hFc is 3.5-18 ng/mL.
• Molecular Mass: The recombinant Rat MET/Fc is a disulfide-linked homodimer. The reduced monomer comprises 1149 amino acids and has a predicted molecular mass of 128.1 kDa. The apparent molecular mass of the protein is approximately 97 and 39 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Met met proto-oncogene [ Rattus norvegicus ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene; hepatocyte growth factor receptor; c-Met; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; Hgfr
• Gene ID: 24553
• mRNA Refseq: NM_031517
• Protein Refseq: NP_113705