Recombinant Human AMIGO3, His-tagged

• Cat.No.: AMIGO3-29H
• Product Overview: Recombinant Human Amphoterin-Induced Protein 3/AMIGO3 produced by transfected human cells is a secreted protein with sequence (Thr20-Thr383) of Human AMIGO3 fused with a polyhistidine tag at the C-terminus.

Specification

• Description: Amphoterin-Induced Gene and ORF 3 (AMIGO3) is a member of the AMIGO family. AMIGO family proteins are cell adhesion molecules, which exhibits homophilic and heterophilic binding properties, plays an important role in neuronal axon tract development. AMIGO3 is a type I transmembrane proteins, includes six leucine-rich repeats (CRRs) and Ig domain in the extracellular domains. AMIGO3 can be expressed and detected in all tissues, may mediates heterophilic cell-cell interaction, contributes to signal transduction through its intracellular domain.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2
• AA Sequence: TPDSEGFPPRALHNCPYKCICAADL LSCTGLGLQDVPAELPAATADLDLS HNALQRLRPGWLAPL FQLRALHLDHNELDALGRGVFVNAS GLRLLDLSSNTLRALGRHDLDGLGA LEKLLLFNNRLVHLD EHAFHGLRALSHLYLGCNELASFSF DHLHGLSATHLLTLDLSSNRLGHIS VPELAALPAFLKNGL YLHNNPLPCDCRLYHLLQRWHQRGL SAVRDFAREYVCLAFKVPASRVRFF QHSRVFENCSSAPAL GLERPEEHLYALVGRSLRLYCNTSV PAMRIAWVSPQQELLRAPGSRDGSI AVLADGSLAIGNVQE QHAGLFVCLATGPRLHHNQTHEYNV SVHFPRPEPEAFNTVDHHHHHH
• Endotoxin: Less than 0.1 ng/μg (1 IEU/μg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in 1X PBS.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Gene Information

• Gene Name: AMIGO3 adhesion molecule with Ig-like domain 3 [ Homo sapiens ]
• Official Symbol: AMIGO3
• Synonyms: AMIGO3; adhesion molecule with Ig-like domain 3; amphoterin-induced protein 3; amphoterin induced gene and open reading frame 3; alivin-3; amphoterin-induced gene and ORF 3; AMIGO-3; MGC120552
• Gene ID: 386724
• mRNA Refseq: NM_198722
• Protein Refseq: NP_942015
• UniProt ID: Q86WK7
• Chromosome Location: 3p21

Reviews

12/09/2021

Consistent and reliable results across different experimental conditions.

11/07/2021

High-resolution imaging for detailed protein localization.

01/14/2020

Precise measurement of protein activity and function.

Q&As

What is the tissue-specific expression pattern of AMIGO3 in the central nervous system and other organs?

AMIGO3 exhibits a unique tissue-specific expression pattern, with prominent expression in the central nervous system, particularly in regions involved in neuronal development and synaptic transmission, and lower expression in other organs.

Can modulation of AMIGO3 expression or activity influence neuronal plasticity and regeneration?

Modulation of AMIGO3 expression or activity has the potential to influence neuronal plasticity and regeneration, promoting axonal growth and functional recovery in the context of injury or neurodegenerative conditions.

Does AMIGO3 play a role in the formation and maintenance of myelin sheaths in the peripheral nervous system?

AMIGO3 may play a role in the formation and maintenance of myelin sheaths in the peripheral nervous system, contributing to proper nerve conduction and insulation of axons.

What are the molecular mechanisms underlying the regulation of AMIGO3 expression during brain development and in response to environmental stimuli?

The regulation of AMIGO3 expression during brain development and in response to environmental stimuli involves complex molecular mechanisms, including transcriptional regulation, epigenetic modifications, and signaling pathways that respond to cues from the local microenvironment.

Are there any functional differences between different isoforms of AMIGO3?

Different isoforms of AMIGO3 may have distinct functions, potentially influencing cellular processes such as neurite outgrowth, synaptogenesis, and cell adhesion in a context-dependent manner.

Can targeting AMIGO3 be a potential therapeutic strategy for neurological conditions or neurodegenerative diseases?

Targeting AMIGO3 expression or activity may hold promise as a therapeutic strategy for neurological conditions or neurodegenerative diseases, and further research is needed to understand its precise role and potential therapeutic implications.

What is the impact of AMIGO3 knockout or overexpression on neuronal connectivity and synaptic function?

Knockout or overexpression studies of AMIGO3 have revealed its critical role in neuronal connectivity and synaptic function, affecting processes such as dendritic arborization, synapse formation, and neurotransmitter release.

Does AMIGO3 interact with specific cell adhesion molecules or signaling receptors, and what are the downstream signaling pathways involved?

AMIGO3 interacts with specific cell adhesion molecules and signaling receptors, including members of the immunoglobulin superfamily and receptor tyrosine kinases, activating downstream signaling pathways involved in axonal guidance, cytoskeletal remodeling, and synaptic plasticity.

How does AMIGO3 contribute to neural development and axonal outgrowth?

AMIGO3 plays a crucial role in neural development and axonal outgrowth, promoting the extension and guidance of growing axons during neuronal circuit formation.

Are there any genetic variations in the AMIGO3 gene associated with neurological disorders or cognitive traits?

Genetic variations in the AMIGO3 gene have been associated with neurological disorders, cognitive traits, and susceptibility to certain psychiatric conditions, suggesting a role in disease susceptibility and cognitive function.