||Recombinant Human Granzyme M/GZMM is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Thr24-Ala257) of Human GZMM fused with a polyhistidine tag at the C-terminus.
||Granzyme M is a secreted protein that belongs to the peptidase S1 family of Granzyme subfamily. Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: Granzyme A, Granzyme B, Granzyme H, and Granzyme M. Granzyme M contains one peptidase S1 domain and is highly, constitutively expressed in activated natural killer (NK) cells. Granzyme M cleaves peptide substrates after methionine, leucine, and norleucine. Its physiological substrates include EZR, α-tubulins and the apoptosis inhibitor BIRC5/Survivin. Granzyme M promotes caspase activation and subsequent apoptosis of target cells.
||TQIIGGREVIPHSRPYMASLQRNGSHLCGGVLVHPKWVLTAAHCLAQRMAQLRLVLGLHTLDSPG LTFHIKAAIQHPRYKPVPALENDLALLQLDGKVKPSRTIRPLALPSKRQVVAAGTRCSMAGWGLT HQGGRLSRVLRELDLQVLDTRMCNNSRFWNGSLSPSMVCLAADSKDQAPCKGDSGGPLVCGKGRV LAGVLSFSSRVCTDIFKPPVATAVAPYVSWIRKVTGRSAVDHHHHHH
||Less than 0.1 ng/μg (1 IEU/μg).
||Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.