| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
26-257 aa |
| Form : |
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Bio-activity : |
GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets a-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Human granzyme M is synthesized as a precursor (264 residues) with a signal peptide (residues 1 23), a propeptide (residues 24-25) and a mature chain (residues 26-257). The purified recombinant human Granzyme M consists of residues 26 to 257 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl•HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMM (diluted by assay buffer) to 50 µL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMM, replace with 50 μL assay buffer. Incubated at 25°C for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme M is >80 pmol/min/µg. |
| Molecular Mass : |
26.3kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
