||Recombinant Human Migration and Invasion-Inhibitory Protein/MIIP is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Pro388) of Human MIIP fused with a polyhistidine tag at the C-terminus.
||Migration and Invasion-Inhibitory Protein (MIIP) was initially discovered in a yeast two-hybrid screen for proteins that interact with and inhibit the migration and invasion-promoting protein insulin-like growth factor binding protein 2 (IGFBP2). It is reported that MIIP not only modulates IGFBP2 but also regulates microtubule by binding to and inhibiting HDAC6, a class 2 histone deacetylase that deacetylates α-tubulin, heat-shock protein 90 (HSP90), and cortactin. In addition, MIIP also regulates the mitosis checkpoint, another microtubule-associated process.
||MVEAEELAQLRLLNLELLRQLWVGQDAVRRSVARAASESSLESSSSYNSETPSTPETSSTSLSTS CPRGRSSVWGPPDACRGDLRDVARSGVASLPPANCQHQESLGRPRPHSAPSLGTSSLRDPEPSGR LGDPGPQEAQTSRSILAQQSKLSKPRVTFSEESAVPERSWRLRPYLGYDWIAGSLDTSSSITSQP EAFFSKLQEFRETNKEECICSHPEPQLPGLRESSGSGVEEDHECVYCYRVNRRLFPVPVDPGTPC RLCRTPRDQQGPGTLAQPAHVRVSIPLSILEPPHRYHIHRRKSFDASDTLALPRHCLLGWDIFPP KSEKSSAPRNLDLWSSVSAEAQHQKLSGTSSPFHPASPMQMLPPTPTWSVPQVPRPHVPRQKPVD HHHHHH
||Less than 0.1 ng/μg (1 IEU/μg).
||Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.