Recombinant Human IRF5, His-tagged

• Cat.No.: IRF5-5121H
• Product Overview: Recombinant human IRF5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: IRF5 is a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
• Molecular Mass: 10.7 kDa (101aa) confirmed by MALDI-TOF
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSPPTL QPPTLQPPVV LGPPAPDPSP LAPPPGNPAG FRELLSEVLE PGPLPASLPP AGEQLLPDLL I
• Purity: >85 % by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: IRF5 interferon regulatory factor 5 [ Homo sapiens ]
• Official Symbol: IRF5
• Synonyms: IRF5; interferon regulatory factor 5; IRF-5; SLEB10
• Gene ID: 3663
• mRNA Refseq: NM_001098627
• Protein Refseq: NP_001092097
• MIM: 607218
• UniProt ID: Q13568
• Chromosome Location: 7q32
• Pathway: Apoptosis, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Direct p53 effectors, organism-specific biosystem; Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem; Interferon alpha/beta signaling, organism-specific biosystem; Interferon gamma signaling, organism-specific biosystem
• Function: protein binding; regulatory region DNA binding; sequence-specific DNA binding transcription factor activity