||Recombinant Human CYP3A5 are a well-established source of cDNA-expressed enzymes for studies of metabolism. Baculovirus-insect cell expressed with supplemental cDNA expressed reductase.
||This gene,CYP3A5, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. This cluster includes a pseudogene, CYP3A5P1, which is very similar to CYP3A5. This similarity has caused some difficulty in determining whether cloned sequences represent the gene or the pseudogene. Multiple alternatively spliced transcript variants have been identified for this gene.
||Catalytic assay: testosterone 6b-hydroxylase Representative catalytic activity: 900 pmole/(mg x min)
||Available size: 1 nmole P450 in 0.5 ml Representative total protein concentration: 7 mg/ml Representative P450 content: 250 pmole/mg
||Generally, 50 mM to 100 mM Tris HCl (pH 7.5) or potassium phosphate (pH 7.4) work well. The activity of cytochromes P450 can vary with buffer and its ionic strength.