Recombinant streptavidin

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Recombinant streptavidin

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Cat.No. : Streptavidin-501
Product Overview : Recombinant streptavidin from Streptomyces avidinii, was produced in Escherichia coli.
Description : Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10^-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.
Source : E. coli
Form : Lyophilized in 10 mM potassium phosphate buffer pH 6.5
Molecular Mass : ~53,000 per tetramer.
AA Sequence : MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS
Endotoxin : Less than 1EU/μg of rStreptavidin as determined by LAL method.
Purity : >95% by SDS-PAGE
Unit Definition : >17 U/mg (one unit binds 1 μg D-biotin at pH 8.9)
Usage : This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Stability : 2 years after shipping
Storage : -20 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution : Dissolve with double distilled water
Shipping : Room temperature
Publication : Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin.

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