"Streptavidin" Related Products

Recombinant streptavidin

Cat.No.: Streptavidin-501
Product Overview: Recombinant streptavidin from Streptomyces avidinii, was produced in Escherichia coli.
Description: Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10^-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin.
Source: E. coli
Form: Lyophilized in 10 mM potassium phosphate buffer pH 6.5
Molecular Mass: ~53,000 per tetramer.
AA Sequence: MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS
Endotoxin: Less than 1EU/μg of rStreptavidin as determined by LAL method.
Purity: >95% by SDS-PAGE
Unit Definition: >17 U/mg (one unit binds 1 μg D-biotin at pH 8.9)
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Stability: 2 years after shipping
Storage: -20 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: Dissolve with double distilled water
Shipping: Room temperature

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