Recombinant streptavidin
Cat.No. : | Streptavidin-501 |
Product Overview : | Recombinant streptavidin from Streptomyces avidinii, was produced in Escherichia coli. |
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Description : | Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1×10^-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. |
Source : | E. coli |
Form : | Lyophilized in 10 mM potassium phosphate buffer pH 6.5 |
Molecular Mass : | ~53,000 per tetramer. |
AA Sequence : | MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS |
Endotoxin : | Less than 1EU/μg of rStreptavidin as determined by LAL method. |
Purity : | >95% by SDS-PAGE |
Unit Definition : | >17 U/mg (one unit binds 1 μg D-biotin at pH 8.9) |
Usage : | This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
Stability : | 2 years after shipping |
Storage : | -20 centigrade. Avoid repeated freeze/thaw cycles. |
Reconstitution : | Dissolve with double distilled water |
Shipping : | Room temperature |
Publication : |
Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin. (2017)
Selenium single-wavelength anomalous diffraction de novo phasing using an X-ray-free electron laser. (2016)
Cooperative Allostery and Structural Dynamics of Streptavidin at Cryogenic- and Ambient-temperature (2021)
A Brief Tour of the Apo-Streptavidin Structures at the SSRL and LCLS Facilities (2021)
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Products Types
◆ Recombinant Protein | ||
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◆ Native Protein | ||
Streptavidin-24 | Streptavidin | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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