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Recombinant Rat Cntf

Cat.No.: Cntf-594R
Product Overview: Recombinant Rat Cntf produced in E. coli is approximately 22.7 kDa, a single non-glycosylated polypeptide chain containing 199amino acids.
Description: Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducin tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant rat CNTF containing 199 amino acids and it shares 83% and 95% a.a. sequence identity with human and murine CNTF.
Source: E. coli
Species: Rat
Form: Lyophilized from a 0.2μm filtered concentrated solution in 2 × PBS, pH 7.4, 2 mM β-mercaptoethanol.
Bio-activity: Fully biologically active when compared to standard. The ED 50 determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of >3.3 × 10^4 IU/mg.
Molecular Mass: 22.7 kDa
AA Sequence: AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
Endotoxin: Less than 1 EU/μg of rRtCNTF as determined by LAL method.
Purity: >95% by SDS-PAGE and HPLC analyses.
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name: Cntf ciliary neurotrophic factor [ Rattus norvegicus ]
Official Symbol: Cntf
Synonyms: CNTF; ciliary neurotrophic factor; ciliary neurotropic factor;
Gene ID: 25707
mRNA Refseq: NM_013166
Protein Refseq: NP_037298
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function: ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; growth factor activity; interleukin-6 receptor binding; interleukin-6 receptor binding;

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