Active Recombinant Pig IL-1beta

• Cat.No.: IL-1beta-599P
• Product Overview: Recombinant Pig IL-1beta produced in E. coli is approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Specification

• Species: Pig
• Source: E.coli
• Tag: Non
• Description: Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. Recombinant porcine interleukin-1 beta is a 17 kDa protein containing 153 amino acid residues and it shares 63 % - 70 % a.a. sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL1β.
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 3 % trehalose.
• Bio-activity: Fully biologically active when compared to standard. The ED 50 determined by a cell proliferation assay using murine D10S cells is less than 7.5 ng/ml, corresponding to a specific activity of >1.3 × 10^5 IU/mg.
• Molecular Mass: 17.6 kDa
• AA Sequence: ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP
• Endotoxin: Less than 1 EU/μg of rPoIL-1β as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apport
• Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportion

Gene Information

• Gene Name: IL-1beta interleukin-1 beta [ Sus scrofa (pig) ]
• Official Symbol: IL-1beta
• Synonyms: IL-1beta;interleukin-1 beta
• Gene ID: 396565
• mRNA Refseq: NM_001005149.1
• Protein Refseq: NP_001005149.1
• Chromosome Location: chromosome: 3
• Pathway: African trypanosomiasis;Alzheimer"s disease;Amoebiasis

Citation

Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation

Journal: Clinics    PubMed ID: 24519204    Data: 2014/2/1

Authors: Luiz Felipe Lopes Araujo, Arthur Rodrigo Ronconi Holand, Cristiano Feijó Andrade

Article Snippet:After the samples were thawed, a 96-well plate was coated with monoclonal antibodies against TNF-α and IL-1β.After the samples were thawed, a 96-well plate was coated with monoclonal antibodies against TNF-α and IL-1β.. The wells were filled with either 100 μl of homogenized lung (diluted 1∶2), 100 μl of positive or negative controls, or 100 μl of recombinant TNF-α or IL-1β at concentrations established by the manufacturer (Creative BioMart, NY, USA).. Then, 100 μl of polyclonal anti-TNF-α and anti-IL-1β conjugated to peroxidase was added to the wells, and the samples were incubated for 3 h at room temperature.Then, 100 μl of polyclonal anti-TNF-α and anti-IL-1β conjugated to peroxidase was added to the wells, and the samples were incubated for 3 h at room temperature.