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Recombinant Flagellin protein

Cat.No. : Flagellin-661
Product Overview : Recombinant Flagellin protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
  • Related Products
Description : Flagellin arranges itself in a hollow cylinder to form the filament in bacterial flagellum. It is the principal substituent of bacterial flagellum, and is present in large amounts on nearly all flagellated bacteria. Mammals often have acquired immune responses (T-cell and antibody responses) to flagellated bacterium and the plant defense mechanisms can be activated by the conserved N-terminal part of flagellin.
Source : E.coli
Species : bacterial flagellum
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Molecular Mass : Approximately 52.7 kDa, a single non-glycosylated polypeptide chain containing 503 amino acids, with Leu, Glu and 6 × His at C-terminus.
Protein length : 503
AA Sequence : MAQVINTNSLSLLTQNNLNKSQSAL GTAIERLSSGLRINSAKDDAAGQAI ANRFTANIKGLTQASRNANDGISIA QTTEGALNEINNNLQRVRELAVQSA NSTNSQSDLDSIQAEITQRLNEIDR VSGQTQFNGVKVLAQDNTLTIQVGA NDGETIDIDLKQINSQTLGLDTLNV QQKYKVSDTAATVTGYADTTIALDN STFKASATGLGGTDQKIDGDLKFDD TTGKYYAKVTVTGGTGKDGYYEVSV DKTNGEVTLAGGATSPLTGGLPATA TEDVKNVQVANADLTEAKAALTAAG VTGTASVVKMSYTDNNGKTIDGGLA VKVGDDYYSATQNKDGSISINTTKY TADDGTSKTALNKLGGADGKTEVVS IGGKTYAASKAEGHNFKAQPDLAEA AATTTENPLQKIDAALAQVDTLRSD LGAVQNRFNSAITNLGNTVNNLTSA RSRIEDSDYATEVSNMSRAQILQQA GTSVLAQANQVPQNVLSLLRLEHHH HHH
Endotoxin : Less than 1 EU/µg of rFlagellin, His as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Flagellin
Official Symbol : Flagellin
Gene ID : 1253480
Protein Refseq : NP_460912.1
UniProt ID : P06179

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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