Recombinant Borrelia garinii p14 flagellin Protein, His-tagged

• Cat.No.: Fla-012B
• Product Overview: Recombinant Borrelia garinii p14 antigen produced from the internal fragment (amino acids 197-274) of the 41 kDa flagellin protein. Contains a His-tag at the N-terminus. Molecular weight calculated from the amino acid sequence is 16.4 kDa.

Specification

• Source: E. coli
• Species: Borrelia burgdorferi
• Tag: His
• Form: Purified, Liquid
• Molecular Mass: 16.4 kDa
• Protein length: amino acids 197-274
• Purity: > 95% pure (SDS-PAGE)
• Applications: Suitable for use in ELISA (3-11 µg/mL), Lateral Flow assays, Dot Blot and Western Blot. It has also been used as a positive control in ELISA, CLIA and Lateral Flow assays. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
• Storage: Store at -20 to -80centigrade. Aliquot to avoid multiple freeze/thaw cycles.
• Concentration: 1.307 mg/mL (Bradford Method)
• Storage Buffer: 20 mM Phosphate, pH 8.0, 1 M NaCl, 0.1% Polyoxyethylene (10) Tridecylether.