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Recombinant Human AKT3, GST-tagged, Active

Cat.No.: AKT3-104H
Product Overview: Recombinant full-length human AKT3 (G171R) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description: AKT3 or Protein Kinase B gamma (PKBgamma) is a serine/threonine kinase that is a member of the AKT family. AKT3 is activated in cells exposed to diverse stimuli such as hormones, growth factors, and extracellular matrix components. AKT3 phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis, and proliferation. Recent evidence indicates that AKT3 is frequently overexpressed in many types of human cancers including breast and prostate.
Source: Sf9 insect cells using baculovirus
Species: Human
Tag: GST
Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity: The specific activity of AKT3 was determined to be 140 nmol /min/mg
Molecular Mass: ~85 kDa
Purity: >95% by densitometry
Applications: Kinase Assay, Western Blot
Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration: 0.1 μg/μl
Gene Name: AKT3 v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) [ Homo sapiens ]
Official Symbol: AKT3
Synonyms: AKT3; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); RAC-gamma serine/threonine-protein kinase; PKBG; PRKBG; RAC gamma; PKB gamma; RAC-gamma serine/threonine protein kinase; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma; DKFZp434N0250;
Gene ID: 10000
mRNA Refseq: NM_001206729
Protein Refseq: NP_001193658
MIM: 611223
UniProt ID: Q9Y243
Chromosome Location: 1q44
Pathway: AKT phosphorylates targets in the cytosol, organism-specific biosystem; AKT phosphorylates targets in the nucleus, organism-specific biosystem; AKT-mediated inactivation of FOXO1A, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem;
Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity;

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