Recombinant Human UBE2U, GST-tagged

• Cat.No.: UBE2U-147H
• Product Overview: Recombinant full-length human UBE2U was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: UBE2U, or Ubiquitin-conjugating enzyme E2 U, belongs to the ubiquitin-conjugating enzyme family. In the global yeast two-hybrid (Y2H) screens to study the specificity of interactions between catalytic domains of the human E2s with RING-type E3s, the analysis showed the UBE2U had a high-quality and physical interactions with a few E3 ligases.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~52 kDa
• Purity: >80% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: UBE2U ubiquitin-conjugating enzyme E2U (putative) [ Homo sapiens ]
• Official Symbol: UBE2U
• Synonyms: UBE2U; ubiquitin-conjugating enzyme E2U (putative); ubiquitin-conjugating enzyme E2 U; MGC35130; ubiquitin-protein ligase U; ubiquitin carrier protein U; RP4-636O23.1
• Gene ID: 148581
• mRNA Refseq: NM_152489
• Protein Refseq: NP_689702
• UniProt ID: Q5VVX9
• Chromosome Location: 1p31.3
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; ubiquitin-protein ligase activity