Recombinant Human TET1, GST-tagged

• Cat.No.: TET1-235H
• Product Overview: Recombinant human TET1 (1598-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: TET1 Protein or tet methylcytosine dioxygenase 1 is the original member of a family of methylcytosine dioxygenases that perform several steps required for cytosine demethylation and gene activation. TET proteins have potential roles in epigenetic regulation through modification of 5mC to hmC. TET1 plays a main role in modulating DNA methylation levels at CpG-rich promoters and also helps in promoting transcription of pluripotency factors as well as participating in the repression of Polycomb-targeted developmental regulators.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~110 kDa
• Purity: >90% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: TET1?tet methylcytosine dioxygenase 1 [?Homo sapiens?(human) ]
• Official Symbol: TET1
• Synonyms: TET1; LCX; CXXC6; bA119F7.1; tet methylcytosine dioxygenase 1; methylcytosine dioxygenase TET1; CXXC finger 6; tet oncogene 1; CXXC zinc finger 6; ten-eleven translocation-1; CXXC-type zinc finger protein 6; ten-eleven translocation 1 gene protein; leukemia-associated protein with a CXXC domain; EC 1.14.11.n2
• Gene ID: 80312
• mRNA Refseq: NM_030625
• Protein Refseq: ?NP_085128
• MIM: 607790
• UniProt ID: Q8NFU7
• Chromosome Location: 10q21
• Function: iron ion binding; methylcytosine dioxygenase activity; structure-specific DNA binding