Recombinant Human UBA2, GST-tagged

• Cat.No.: UBA2-253H
• Product Overview: Recombinant full-length human UBA2 (SAE2) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: UBA2 (SAE2) or ubiquitin-like modifier activating enzyme 2 forms a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. The posttranslational modification of proteins by the addition of the small protein SUMO or sumoylation, regulates protein structure and intracellular localization. UBA2 functions in SUMO1 activation in a manner analogous to the single E1 ubiquitin-activating enzymes. The inactivation of UBA2 may be a therapeutic strategy in MYC-driven cancers.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~115 kDa
• Purity: >85% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: UBA2 ubiquitin-like modifier activating enzyme 2 [ Homo sapiens ]
• Official Symbol: UBA2
• Synonyms: UBA2; ubiquitin-like modifier activating enzyme 2; SAE2, SUMO1 activating enzyme subunit 2; SUMO-activating enzyme subunit 2; ARX; FLJ13058; HRIHFB2115; ubiquitin activating enzyme E1 homolog (yeast); SUMO1 activating enzyme subunit 2; SUMO-1 activating enzyme subunit 2; ubiquitin-like 1-activating enzyme E1B; anthracycline-associated resistance ARX; UBA2, ubiquitin-activating enzyme E1 homolog; SAE2
• Gene ID: 10054
• mRNA Refseq: NM_005499
• Protein Refseq: NP_005490
• MIM: 613295
• UniProt ID: Q9UBT2
• Chromosome Location: 19q13.11
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem
• Function: ATP binding; SUMO activating enzyme activity; enzyme activator activity; ligase activity; metal ion binding; nucleotide binding; protein binding; protein heterodimerization activity; transcription factor binding