Recombinant Mouse Kdm5b, GST-tagged

• Cat.No.: Kdm5b-261M
• Product Overview: Recombinant mouse KDM5B (1-753) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: KDM5B is a lysine (K)-specific demethylase 5B which is highly expressed in testis and shows the lowest expression in skeletal muscle. KDM5B is a histone H3 lysine 4 (H3K4) demethylase up-regulated in prostate cancer and associated with androgen receptor where it regulated its transcriptional activity in reporter gene assays. Overexpression of KDM5B in HeLa cells results in loss of tri-, di-, and monomethyl H3K4, but does not alter the methylation status of H3K9 or H3K36. A KDM5B containing highly conserved putative DNA/chromatin binding motif is specifically up-regulated in breast cancer.
• Source: Sf9 insect cells using baculovirus
• Species: Mouse
• Tag: GST
• Form: Recombinant protein stored in 50mM Tis-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~112 kDa
• Purity: >90% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: Kdm5b lysine (K)-specific demethylase 5B [ Mus musculus ]
• Official Symbol: Kdm5b
• Synonyms: KDM5B; lysine (K)-specific demethylase 5B; lysine-specific demethylase 5B; histone demethylase JARID1B; putative DNA/chromatin binding motif 1; jumonji/ARID domain-containing protein 1B; jumonji, AT rich interactive domain 1B (Rbp2 like); PUT1; Plu1; PLU-1; Rb-Bp2; Jarid1b; AW556288; RBBP2H1A; mKIAA4034; D1Ertd202e; 2010009J12Rik; 2210016I17Rik; KIAA4034
• Gene ID: 75605
• mRNA Refseq: NM_152895
• Protein Refseq: NP_690855
• Function: DNA binding; histone demethylase activity (H3-K4 specific); histone demethylase activity (H3-dimethyl-K4 specific); histone demethylase activity (H3-trimethyl-K4 specific); metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; transcription corepressor activity; zinc ion binding