||Recombinant human DKK-3 expressed in CHO cells is a glycoprotein that has a calculated molecular weight of 36.3 kDa and contains 329 amino acid residues.
||This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein.
||Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED50 for this effect is 2.0–4.0 ng/ml.
||APAPTATSAP VKPGPALSYP QEEATLNEMF REVEELMEDT QHKLRSAVEE MEAEEAAAKA SSEVNLANLP PSYHNETNTD TKVGNNTIHV HREIHKITNN QTGQMVFSET VITSVGDEEG RRSHECIIDE DCGPSMYCQF ASFQYTCQPC RGQRMLCTRD SECCGDQLCV WGHCTKMATR GSNGTICDNQ RDCQPGLCCA FQRGLLFPVC TPLPVEGELC HDPASRLLDL ITWELEPDGA LDRCPCASGL LCQPHSHSLV YVCKPTFVGS RDQDGEILLP REVPDEYEVG SFMEEVRQEL EDLERSLTEE MALREPAAAA AALLGGEEI
||Greater than 98% by SDS-PAGE gel and HPLC analyses.