Recombinant Human TOP2A

• Cat.No.: TOP2A-115H
• Product Overview: Recombinant enzyme overexpressed in a proprietary eukaryotic cell line.

Specification

• Description: Topoisomerase II α (TopoIIα) is a gene product with conserved catalytic activities and it promotes the progression of DNA damage. The α isoform is present in proliferating cells.
• Species: Human
• Form: Solution in 10 mM Tris-HCl, pH 7.1, 0.25 M NaCl, 1 mM EDTA, 0.5 mM PMSF, 1 mM 2-mercaptoethanol, 10% glycerol.
• Molecular Mass: mol wt 170 kDa
• Unit Definition: One unit will decatenate 0.2 μg of kinetoplast DNA in 30 minutes at 37°C.
• Storage: Store at −70°C

Gene Information

• Gene Name: TOP2A topoisomerase (DNA) II alpha 170kDa [ Homo sapiens ]
• Official Symbol: TOP2A
• Synonyms: TOP2A; topoisomerase (DNA) II alpha 170kDa; TOP2, topoisomerase (DNA) II alpha (170kD); DNA topoisomerase 2-alpha; DNA gyrase; DNA topoisomerase II, 170 kD; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase II, alpha isozyme; TOP2; TP2A
• Gene ID: 7153
• mRNA Refseq: NM_001067
• Protein Refseq: NP_001058
• MIM: 126430
• UniProt ID: P11388
• Chromosome Location: 17q21-q22
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; G0 and Early G1, organism-specific biosystem; Mitotic G1-G1/S phases, organism-specific biosystem
• Function: ATP binding; DNA binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA-dependent ATPase activity; chromatin binding; drug binding; enzyme binding; histone deacetylase binding; nucleotide binding; protein C-terminus binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase C binding; sequence-specific DNA binding; structure-specific DNA binding; ubiquitin binding