"POLR2F" Related Products


Recombinant Human POLR2F, His-tagged

Cat.No.: POLR2F-154H
Product Overview: POLR2F, 1-127aa, Human, His tag, E.coli
Description: POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source: E.coli
Species: Human
Tag: His-tag
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Molecular Mass: 16.9kDa (150aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
Protein length: 1-127aa
Purity: >95% by SDS - PAGE
Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration: 1.0 mg/ml (determined by BRADFORD assay)
Gene Name: POLR2F polymerase (RNA) II (DNA directed) polypeptide F [ Homo sapiens ]
Official Symbol: POLR2F
Synonyms: POLR2F; polymerase (RNA) II (DNA directed) polypeptide F; DNA-directed RNA polymerases I, II, and III subunit RPABC2; DNA directed RNA polymerase II 14.4 kda polypeptide; HRBP14.4; RPB6; RPC15; RPABC14.4; RPB6 homolog; RNA Polymerase II subunit 14.4 kD; DNA-directed RNA polymerase II subunit F; RNA polymerases I, II, and III subunit ABC2; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; POLRF; RPABC2; RPB14.4;
Gene ID: 5435
mRNA Refseq: NM_021974
Protein Refseq: NP_068809
MIM: 604414
UniProt ID: P61218
Chromosome Location: 22q13.1
Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem;
Function: DNA binding; DNA-directed RNA polymerase activity; contributes_to protein kinase activity;

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