Recombinant Human POLR2F, His-tagged
Cat.No. : | POLR2F-154H |
Product Overview : | POLR2F, 1-127aa, Human, His tag, E.coli |
- Specification
- Gene Information
- Related Products
Description : | POLR2F is the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Recombinant human POLR2F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Source : | E.coli |
Species : | Human |
Tag : | His-tag |
Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT |
Molecular Mass : | 16.9kDa (150aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher) |
Protein length : | 1-127aa |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD |
Purity : | >95% by SDS - PAGE |
Storage : | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
Concentration : | 1.0 mg/ml (determined by BRADFORD assay) |
Gene Name : | POLR2F polymerase (RNA) II (DNA directed) polypeptide F [ Homo sapiens ] |
Official Symbol : | POLR2F |
Synonyms : | POLR2F; polymerase (RNA) II (DNA directed) polypeptide F; DNA-directed RNA polymerases I, II, and III subunit RPABC2; DNA directed RNA polymerase II 14.4 kda polypeptide; HRBP14.4; RPB6; RPC15; RPABC14.4; RPB6 homolog; RNA Polymerase II subunit 14.4 kD; DNA-directed RNA polymerase II subunit F; RNA polymerases I, II, and III subunit ABC2; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; POLRF; RPABC2; RPB14.4; |
Gene ID : | 5435 |
mRNA Refseq : | NM_021974 |
Protein Refseq : | NP_068809 |
MIM : | 604414 |
UniProt ID : | P61218 |
Chromosome Location : | 22q13.1 |
Pathway : | Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem; |
Function : | DNA binding; DNA-directed RNA polymerase activity; contributes_to protein kinase activity; |
Products Types
◆ Recombinant Protein | ||
POLR2F-3334R | Recombinant Rhesus Macaque POLR2F Protein, His (Fc)-Avi-tagged | +Inquiry |
Polr2f-4998M | Recombinant Mouse Polr2f Protein, Myc/DDK-tagged | +Inquiry |
POLR2F-6923M | Recombinant Mouse POLR2F Protein, His (Fc)-Avi-tagged | +Inquiry |
POLR2F-374H | Recombinant Human POLR2F Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
POLR2F-4230R | Recombinant Rat POLR2F Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
POLR2F-3033HCL | Recombinant Human POLR2F 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket