Recombinant Human GLUD1, His-tagged GLUD1-160H

Recombinant Human GLUD1, His-tagged

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Recombinant Human GLUD1, His-tagged

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Cat.No. : GLUD1-160H
Product Overview : GLUD1, 54-558aa, Human, His tag, E.coli
Description : Glutamate dehydrogenase 1, mitochondrial precursor, also known as GLUD1, belongs to the Glu/Leu/Phe/Val dehydrogenases family. GLUD1 has a central role in nitrogen metabolism in plants and animals. GLUD1 is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Mutations in the gene encoding GLUD1 causes hyperinsulinism-hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood. GLUD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Recombinant human GLUD1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
Source : E.coli
Species : Human
Tag : His-tag
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol
Molecular Mass : 58.4 kDa (528aa)
Protein length : 54-558aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSSEAVADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLR TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED FKLQHGSILG FPKAKPYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPIVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY NEAGVTFT
Purity : >80% by SDS - PAGE
Storage : Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration : 1mg/ml (determined by Bradford assay)
Gene Name : GLUD1 glutamate dehydrogenase 1 [ Homo sapiens ]
Official Symbol : GLUD1
Synonyms : GLUD1; glutamate dehydrogenase 1; GLUD; glutamate dehydrogenase 1, mitochondrial; GDH; GDH 1; glutamate dehydrogenase (NAD(P)+); GDH1; MGC132003;
Gene ID : 2746
mRNA Refseq : NM_005271
Protein Refseq : NP_005262
MIM : 138130
UniProt ID : P00367
Chromosome Location : 10q23.2
Pathway : Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; D-Glutamine and D-glutamate metabolism, organism-specific biosystem; D-Glutamine and D-glutamate metabolism, conserved biosystem;
Function : ADP binding; ATP binding; GTP binding; NAD+ binding; glutamate dehydrogenase (NAD+) activity; glutamate dehydrogenase [NAD(P)+] activity; glutamate dehydrogenase [NAD(P)+] activity; identical protein binding; leucine binding; nucleotide binding; oxidoreductase activity; protein binding;

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