Recombinant Human NELFE, His-tagged
| Cat.No. : | NELFE-192H |
| Product Overview : | NELFE, 1-380aa, Human, His tag, E.coli |
- Specification
- Gene Information
- Related Products
| Description : | NELFE is a part of complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins; however, it has not been demonstrated that this protein binds RNA. It contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). Recombinant human NELFE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques |
| Source : | E.coli |
| Species : | Human |
| Tag : | His-tag |
| Form : | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 5mM DTT |
| Molecular Mass : | 45.6kDa (403aa) confirmed by MALDI-TOF |
| Protein length : | 1-380aa |
| AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSMLVIPPG LSEEEEALQK KFNKLKKKKK ALLALKKQSS SSTTSQGGVK RSLSEQPVMD TATATEQAKQ LVKSGAISAI KAETKNSGFK RSRTLEGKLK DPEKGPVPTF QPFQRSISAD DDLQESSRRP QRKSLYESFV SSSDRLRELG PDGEEAEGPG AGDGPPRSFD WGYEERSGAH SSASPPRSRS RDRSHERNRD RDRDRERDRD RDRDRDRERD RDRDRDRDRD RERDRDRERD RDRDREGPFR RSDSFPERRA PRKGNTLYVY GEDMTPTLLR GAFSPFGNII DLSMDPPRNC AFVTYEKMES ADQAVAELNG TQVESVQLKV NIARKQPMLD AATGKSVWGS LAVQNSPKGC HRDKRTQIVY SDDVYKENLV DGF |
| Purity : | >90% by SDS - PAGE |
| Storage : | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| Concentration : | 0.25 mg/ml (determined by BRADFORD assay) |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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