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Recombinant Human CD14, His-tagged

Cat.No.: CD14-204H
Product Overview: CD14, 20- 349aa, Human, His tag, E.coli
Description: CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. Recombinant human CD14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source: E.coli
Species: Human
Tag: His-tag
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.15M NaCl, 1mM DTT
Molecular Mass: 37.9 kDa (353aa) confirmed by MALDI-TOF
Protein length: 20- 349aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSTTPEPCE LDDEDFRCVC NFSEPQPDWS EAFQCVSAVE VEIHAGGLNL EPFLKRVDAD ADPRQYADTV KALRVRRLTV GAAQVPAQLL VGALRVLAYS RLKELTLEDL KITGTMPPLP LEATGLALSS LRLRNVSWAT GRSWLAELQQ WLKPGLKVLS IAQAHSPAFS CEQVRAFPAL TSLDLSDNPG LGERGLMAAL CPHKFPAIQN LALRNTGMET PTGVCAALAA AGVQPHSLDL SHNSLRATVN PSAPRCMWSS ALNSLNLSFA GLEQVPKGLP AKLRVLDLSC NRLNRAPQPD ELPEVDNLTL DGNPFLVPGT ALPHEGSMNS GVV
Purity: >90% by SDS - PAGE
Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration: 0.5mg/ml (determined by Bradford assay)
Gene Name: CD14 CD14 molecule [ Homo sapiens ]
Official Symbol: CD14
Synonyms: CD14; CD14 molecule; CD14 antigen; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein;
Gene ID: 929
mRNA Refseq: NM_000591
Protein Refseq: NP_000582
MIM: 158120
UniProt ID: P08571
Chromosome Location: 5q22-q32
Pathway: Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; LPS transferred from LBP carrier to CD14, organism-specific biosystem;
Function: lipopolysaccharide binding; lipoteichoic acid binding; opsonin receptor activity; peptidoglycan receptor activity;

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