"ATG3" Related Products

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Recombinant Human ATG3, His-tagged

Cat.No.: ATG3-222H
Product Overview: ATG3, 1-314aa, Human, His tag, E.coli
Description: Ubiquitin-like-conjugating enzyme ATG3 isoform 1, also known as ATG3, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. ATG3 catalyzes the formation of the Atg8-phosphatidylethanolamine (ATG-PE) conjugate, a reaction that is essential for autophagy. Recombinant human ATG3 protien, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source: E.coli
Species: Human
Tag: His-tag
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
Molecular Mass: 38.3 kDa (337aa) confirmed by MALDI-TOF
Protein length: 1-314aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQNVINT VKGKALEVAE YLTPVLKESK FKETGVITPE EFVAAGDHLV HHCPTWQWAT GEELKVKAYL PTGKQFLVTK NVPCYKRCKQ MEYSDELEAI IEEDDGDGGW VDTYHNTGIT GITEAVKEIT LENKDNIRLQ DCSALCEEEE DEDEGEAADM EEYEESGLLE TDEATLDTRK IVEACKAKTD AGGEDAILQT RTYDLYITYD KYYQTPRLWL FGYDEQRQPL TVEHMYEDIS QDHVKKTVTI ENHPHLPPPP MCSVHPCRHA EVMKKIIETV AEGGGELGVH MYLLIFLKFV QAVIPTIEYD YTRHFTM
Purity: >85% by SDS - PAGE
Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration: 0.5 mg/ml (determined by Bradford assay)
Gene Name: ATG3 ATG3 autophagy related 3 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol: ATG3
Synonyms: ATG3; ATG3 autophagy related 3 homolog (S. cerevisiae); APG3 autophagy 3 like (S. cerevisiae) , APG3L; ubiquitin-like-conjugating enzyme ATG3; DKFZp564M1178; FLJ22125; MGC15201; PC3 96; hApg3; 2610016C12Rik; autophagy-related protein 3; APG3; APG3L; PC3-96; APG3-LIKE;
Gene ID: 64422
mRNA Refseq: NM_022488
Protein Refseq: NP_071933
MIM: 609606
UniProt ID: Q9NT62
Chromosome Location: 3q13.2
Pathway: Regulation of autophagy, organism-specific biosystem; Regulation of autophagy, conserved biosystem; Senescence and Autophagy, organism-specific biosystem;
Function: Atg12 ligase activity; Atg8 ligase activity; enzyme binding; ligase activity; protein binding; small conjugating protein ligase activity;

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