Active Recombinant Human KSR2 Mutant (R676S), GST-tagged

• Cat.No.: KSR2-83H
• Product Overview: Recombinant human KSR2 (R676S) (554-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: KSR2 or kinase suppressor of Ras 2 specifically regulates the activity of MEKK3 and COT but not other members of the MAP3K family. KSR interacts with a regulatory Raf molecule in cis to induce a conformational switch of MEK, facilitating MEK"s phosphorylation by a separate catalytic Raf molecule in trans.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity was determined to be 48 nmol/min/mg
• Molecular Mass: ~72 kDa
• Purity: >70% by densitometry.
• Applications: Kinase Assay
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: KSR2 kinase suppressor of ras 2 [ Homo sapiens ]
• Official Symbol: KSR2
• Synonyms: KSR2; kinase suppressor of ras 2; kinase suppressor of Ras 2; FLJ25965
• Gene ID: 283455
• mRNA Refseq: NM_173598
• Protein Refseq: NP_775869
• MIM: 610737
• UniProt ID: Q6VAB6
• Chromosome Location: 12q24.22-q24.23
• Function: ATP binding; metal ion binding; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups