||Recombinant Human MSH6 as expressed as 6*his-tagged fusion protein by E.Coli and purified by Ni-sepharose.
||This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described.
||The protein is stored in PBS buffer at -20 oC. Repeated freeze-thaw cycles should be avoided.
||1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.