Active Recombinant Human MEPE, His-tagged

• Cat.No.: MEPE-5688H
• Product Overview: Recombinant Human MEPE (Pro18-Asp525 & Phe20-Asp525 & Gln21-Asp525), fused with a C-terminal 10-His tag, was expressed in NS0 cells.

Specification

• Description: Matrix extracellular phosphoglycoprotein (Osteoblast/osteocyte factor 45) is a protein that in humans is encoded by the MEPE gene. A conserved RGD motif is found in this protein, and this is potentially involved in integrin recognition.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Pro18, Phe20 & Gln21
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. rhMEPE, immobilized at 2 µg/mL (100 µL/well), can induce more than 60% of ATDC5 cell adhesion.
• Molecular Mass: Predicted Molecular Mass: 57 kDa SDS-PAGE: 82 kDa, reducing conditions
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.

Gene Information

• Gene Name: MEPE matrix extracellular phosphoglycoprotein [ Homo sapiens (human) ]
• Official Symbol: MEPE
• Synonyms: MEPE; OF45; matrix extracellular phosphoglycoprotein; osteoblast/osteocyte factor 45; matrix, extracellular phosphoglycoprotein with ASARM motif (bone)
• Gene ID: 56955
• mRNA Refseq: NM_001184694
• Protein Refseq: NP_001171623
• MIM: 605912
• UniProt ID: Q9NQ76
• Chromosome Location: 4q21.1
• Function: extracellular matrix structural constituent; protein binding