"Lep" Related Products

Expand
Expand
Expand

Recombinant Rat Leptin, Antagonist Triple Mutant, Mono-PEG

Cat. No.: Lep-28R
Product Overview: Recombinant Rat Leptin Antagonist Triple Mutant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. The Rat Leptin antagonist was mutated, resulting in L39A/D40A/F41A mutant. The Rat Leptin antagonist is bound to 20 kDa mono-PEG at N-terminus, resulting in 35.6 kDa. The Rat Leptin triple anatagonist runs as a 48 kDa. Leptin Antagonist Triple Mutant Rat Recombinant was purified by proprietary chromatographic techniques.
Description: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
Source: Escherichia Coli.
Physical Appearance: White lyophilized (freeze-dried) powder.
Purity: Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
Formulation: The The Rat Leptin triple anatagonist was lyophilized from a concentrated (0.65 mg/ml) solution with 0.003 mM NaHCO3.
Solubility: It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Rat Recombinant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9. It is not less than 100 µg/ml, which can then be further diluted with other aqueous solutions.
Specific Activity: Leptin Antagonist Triple Mutant Rat Recombinant half-life in circulation after SC injection was over 20 hours. Leptin Antagonist Triple Mutant Rat Recombinant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Leptin Antagonist Triple Mutant Rat Recombinant in vitro activity is 5-6 fold lower than the non-pegylated antagonist, though in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist), resulting mainly from increased food intake.
Protein Content: Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.2 as the extinction coefficient for a 0.1% (1 mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Stability: Lyophilized Leptin Antagonist Triple Mutant Rat Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4°Cor even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Gene Name: Lep leptin [ Rattus norvegicus ]
Synonyms: Lep; leptin; OB; obese; Obesity (murine homolog, leptin)
Gene ID: 25608
mRNA Refseq: NM_013076
Protein Refseq: NP_037208
UniProt ID: P50596
Chromosome Location: 4q22
Pathway: Adipocytokine signaling pathway; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction
Function: cytokine activity; growth factor activity; hormone activity; protein binding; peptide hormone receptor binding

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.