Recombinant Anopheles gambiae TDO, His-tagged


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Recombinant Anopheles gambiae TDO, His-tagged

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Cat.No. : TDO-85A
Product Overview : Full-length TDO (Anopheles gambiae) is fused at the N-terminus to a His-tag.
Description : In enzymology, a tryptophan 2,3-dioxygenase (EC is an enzyme that catalyzes the chemical reactionL-tryptophan + O2 = N-formyl-L-kynurenineThus, the two substrates of this enzyme are L-tryptophan and O2, whereas its product is N-formyl-L-kynurenine. This enzyme participates in tryptophan metabolism.
Source : E. coli
Species : Anopheles gambiae
Tag : His
Form : Liquid. In 50mM sodium phosphate pH 8.0, containing 0.15M sodium chloride, 0.2M imidazole and 10% glycerol.
Bio-activity : Specific activity towards L-Trp: 87.0μmol/hr per mg. TDO catalyzes the oxidative opening of the L-tryptophan indole moiety to yield N-formylkynurenine (NFK).
Molecular Mass : ~47.9kDa
Endotoxin : N/A
Purity : ≥98% (SDS-PAGE)
Applications : Can be used to test potential kynurenine pathway inhibitors in the mosquito, by continuously monitoring the rate of NFK formation at 321nm (ε=3152/M cm) in in vitro spectrophotometric assays.
Stability : Stable for at least 6 months after receipt when stored at -80°C.
Storage : Short Term Storage: -20°CLong Term Storage: -80°CAfter opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Concentration : 0.5mg/ml (Lot specific)
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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