||Recombinant full-length human TRIB1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
||TRIB1 or tribbles homolog 1 protein encodes one of three members of the Tribbles family that share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. TRIB1 is highly expressed in skeletal muscle, thyroid, pancreas, peripheral blood leukocytes, and bone marrow. The overexpression of TRIB1 may be functionally important consequence of 8q24 amplicons in AML and MDS. TRIB1 is a p65-interacting negative regulator of NF-kappa-B-dependent transcription.
||Sf9 insect cells using baculovirus
||50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
||Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.