Active Recombinant Human CHI3L2, His-tagged

• Cat.No.: CHI3L2-6543H
• Product Overview: Recombinant Human CHI3L2 (Tyr27-Leu390), fused with a C-terminal 10-His tag, was expressed in Mouse NSO Cells.

Specification

• Description: The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Tyr27
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells.When 5 x 104 cells per well are added to rhCHI3L2 coated plates for 30 minutes at 37 °C, cell adhesion is enhanced in a dose dependent manner with typical ED50 range of 0.7 - 2.8 μg/mL. Opitimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Predicted Molecular Mass:42.2 kDaSDS-PAGE:40 kDa, reducing conditions
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: CHI3L2?chitinase 3-like 2 [?Homo sapiens?(human) ]
• Official Symbol: CHI3L2
• Synonyms: CHI3L2; CHIL2; YKL39; YKL-39; chitinase 3-like 2; chitinase-3-like protein 2; chondrocyte protein 39
• Gene ID: 1117
• mRNA Refseq: NM_004000
• Protein Refseq: NP_003991
• MIM: 601526
• UniProt ID: Q15782
• Chromosome Location: 1p13.3
• Function: chitin binding; NOT chitinase activity