Recombinant Human PHD finger protein 5A, His-tagged

• Cat.No.: PHF5A-213H
• Product Overview: PHF5A, 1-110aa, Human, His tag, E.coli

Specification

• Description: PHF5A is a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron"s branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. PHF5A contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. Recombinant human PHF5A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 14.8kDa (133aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAKHHPD LIFCRKQAGV AIGRLCEKCD GKCVICDSYV RPCTLVRICD ECNYGSYQGR CVICGGPGVS DAYYCKECTI QEKDRDGCPK IVNLGSSKTD LFYERKKYGF KKR
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: PHF5A PHD finger protein 5A [ Homo sapiens ]
• Official Symbol: PHF5A
• Synonyms: PHF5A; PHD finger protein 5A; PHD finger-like domain-containing protein 5A; bK223H9.2; INI; MGC1346; Rds3; SAP14b; SF3b14b; PHD-finger 5a; PHD finger-like domain protein 5A; splicing factor 3B associated 14 kDa protein; splicing factor 3B-associated 14 kDa protein
• Gene ID: 84844
• mRNA Refseq: NM_032758
• Protein Refseq: NP_116147
• UniProt ID: Q7RTV0
• Chromosome Location: 22q13.2
• Pathway: Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Spliceosome, U2-snRNP, organism-specific biosystem; mRNA Splicing, organism-specific biosystem; mRNA Splicing - Major Pathway, organism-specific biosystem
• Function: DNA binding; sequence-specific DNA binding transcription factor activity