Recombinant Human steroid receptor RNA activator 1, His-tagged

• Cat.No.: SRA1-357H
• Product Overview: SRA1, 90-236aa, Human, His tag, E.coli

Specification

• Description: Steroid receptor RNA activator 1, also known as SRA1, exists as both a RNA transcript and as stably expressed proteins. Also this protein mediates transcriptional coactivation of steroid receptors ligand-dependently through the steroid-binding domain (AF-2). SRA1 enhances cellular proliferation and differentiation and promotes apoptosis in vivo. It may play a role in tumorigenesis. Recombinant human SRA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
• Molecular Mass: 18.7 kDa (170aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSVGSGPAS GVEPTSFPVE SEAVMEDVLR PLEQALEDCR GHTRKQVCDD ISRRLALLQE QWAGGKLSIP VKKRMALLVQ ELSSHRWDAA DDIHRSLMVD HVTEVSQWMV GVKRLIAEKR SLFSEEAANE EKSAATAEKN HTIPGFQQAS
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: Lanz R B., et al. (1999) Cell. 97:17-27Watanabe M., et al. (2001) EMBO J. 20: 1341-1352.

Gene Information

• Gene Name: SRA1 steroid receptor RNA activator 1 [ Homo sapiens ]
• Official Symbol: SRA1
• Synonyms: SRA1; steroid receptor RNA activator 1; steriod receptor RNA activator 1; SRA; STRAA1; steroid receptor coactivator; steroid receptor RNA activator protein; steroid receptor RNA activator 1 (complexes with NCOA1); SRAP; pp7684; MGC87674
• Gene ID: 10011
• mRNA Refseq: NM_001035235
• Protein Refseq: NP_001030312
• MIM: 603819
• UniProt ID: Q9HD15
• Chromosome Location: 5q31
• Function: DNA binding; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; receptor activity; thyroid hormone receptor activator activity; transcription coactivator activity