| Description : |
AN1-type zinc finger protein 1 isoform a, also known as ZFAND1, belongs to the Zinc-finger proteins. Zinc -finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZFAND1 is a 268 amino acid protein that contains 2 AN1-type zinc fingers, which are often found in proteins that contain an ubiquitin-like domain and therefore play a role in the ubiquitination pathway. ZFAND1 contains six conserved cysteines and two histidines and have a dimetal (zinc)-bound alpha/beta fold. There are two isoforms of ZFAND1 that are produced as a result of alternative splicing events. Recombinant human ZFAND1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Source : |
E.coli |
| Species : |
Human |
| Tag : |
His-tag |
| Form : |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
| Molecular Mass : |
33.2kDa (291aa) confirmed by MALDI-TOF |
| AA Sequence : |
MGSSHHHHHH SSGLVPRGSH MGSMAELDIG QHCQVEHCRQ RDFLPFVCDD CSGIFCLEHR SRESHGCPEV TVINERLKTD QHTSYPCSFK DCAERELVAV ICPYCEKNFC LRHRHQSDHE CEKLEIPKPR MAATQKLVKD IIDSKTGETA SKRWKGAKNS ETAAKVALMK LKMHADGDKS LPQTERIYFQ VFLPKGSKEK SKPMFFCHRW SIGKAIDFAA SLARLKNDNN KFTAKKLRLC HITSGEALPL DHTLETWIAK EDCPLYNGGN IILEYLNDEE QFCKNVESYL E |
| Purity : |
>90% by SDS - PAGE |
| Storage : |
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| Concentration : |
0.5 mg/ml (determined by Bradford assay) |
