Recombinant Human general transcription factor IIH, polypeptide 5, His-tagged

• Cat.No.: GTF2H5-659H
• Product Overview: GTF2H5, 1-71aa Human, His tag, E.coli

Specification

• Description: GTF2H5 is a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating cellular levels of TFIIH. Mutations in this gene result in trichothiodystrophy, complementation group A. Recombinant human GTF2H5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid, In Phosphate buffered saline (pH7.4) containing 10% glycerol
• Molecular Mass: 10.4 kDa (94aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVNVLKG VLIECDPAMK QFLLYLDESN ALGKKFIIQD IDDTHVFVIA ELVNVLQERV GELMDQNAFS LTQK
• Purity: >95 % by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GTF2H5 general transcription factor IIH, polypeptide 5 [ Homo sapiens (human) ]
• Official Symbol: GTF2H5
• Synonyms: GTF2H5; TTD; TFB5; TTDA; TFIIH; TTD-A; TGF2H5; C6orf175; bA120J8.2; general transcription factor IIH, polypeptide 5; general transcription factor IIH subunit 5; TFB5 ortholog; TFIIH basal transcription factor complex TTDA subunit; TFIIH basal transcription factor complex TTD-A subunit
• Gene ID: 404672
• mRNA Refseq: NM_207118
• Protein Refseq: NP_997001
• MIM: 608780
• UniProt ID: Q6ZYL4
• Chromosome Location: 6q25.3
• Pathway: Basal transcription factors; Holo-TFIIH complex; Nucleotide excision repair