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Recombinant Human ubiquitin-conjugating enzyme E2G 1, His-tagged

Cat.No.: UBE2G1-667H
Product Overview: UBE2G1, 1-170aa Human, His tag, E.coli
Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UE2G1 is a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins. The protein may be involved in degradation of muscle-specific proteins. Recombinant human UBE2G1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source: E.coli
Species: Human
Tag: His-tag
Form: Liquid. In Phosphate buffered saline (pH7.4) containing, 30% glycerol, 1mM DTT
Molecular Mass: 21.9kDa (193aa) confirmed by MALDI-TOF
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTELQSA LLLRRQLAEL NKNPVEGFSA GLIDDNDLYR WEVLIIGPPD TLYEGGVFKA HLTFPKDYPL RPPKMKFITE IWHPNVDKNG DVCISILHEP GEDKYGYEKP EERWLPIHTV ETIMISVISM LADPNGDSPA NVDAAKEWRE DRNGEFKRKV ARCVRKSQET AFE
Purity: >95% by SDS - PAGE
Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration: 0.5 mg/ml (determined by Bradford assay)
Gene Name: UBE2G1 ubiquitin-conjugating enzyme E2G 1 [ Homo sapiens ]
Official Symbol: UBE2G1
Synonyms: UBE2G1; ubiquitin-conjugating enzyme E2G 1; UBE2G, ubiquitin conjugating enzyme E2G 1 (homologous to C. elegans UBC7) , ubiquitin conjugating enzyme E2G 1 (UBC7 homolog, C. elegans) , ubiquitin conjugating enzyme E2G 1 (UBC7 homolog, yeast); ubiquitin-conjugating enzyme E2 G1; UBC7; ubiquitin-protein ligase G1; ubiquitin carrier protein G1; ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, yeast); ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans); ubiquitin-conjugating enzyme E2G 1 (homologous to C. elegans UBC7); E217K; UBE2G;
Gene ID: 7326
mRNA Refseq: NM_003342
Protein Refseq: NP_003333
MIM: 601569
UniProt ID: P62253
Chromosome Location: 17p13.2
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Parkinsons disease, organism-specific biosystem;
Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity;

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