Recombinant Human B3GAT3 protein, His-tagged
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|Product Overview :||Recombinant Human B3GAT3 (Glu72-Val335) protein was fused to His-tag at N-terminus and expressed in E.coli.|
|Description :||Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 (B3GAT3), a member of the glycosyltransferase 43 family, is also known as beta-1,3-glucuronyltransferase 3, glucuronosyltransferase I (GlcAT-I) and UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase (GlcUAT-I). B3GAT3 plays a role in glycosaminoglycans biosynthesis and be involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. B3GAT3 can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Furthermore,B3GAT3 shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc.|
|Predicted N Terminal :||Glu72|
|Form :||Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 30.2 kDa. The protein migrates as 32 kDa under reducing (R) condition (SDS-PAGE).|
|Protein length :||Glu72-Val335|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>92% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of B3GAT3-228H :||
Human B3GAT3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.
|Gene Name :||B3GAT3|
|Official Symbol :||B3GAT3|
|Synonyms :||B3GAT3; beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I); galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3; GlcAT I; glcUAT-I; Sqv-8-like protein; UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase; GLCATI|
|Gene ID :||26229|
|mRNA Refseq :||NM_012200|
|Protein Refseq :||NP_036332|
|UniProt ID :||O94766|
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