|Product Overview:||Recombinant Human MAPK13(Met1-Leu365), activated in vitro by MAP2K6 (10422-H20B1) and fused with GST tag at N-terminal was expressed in Insect cells.|
|Description:||The p38 family of mitogen-activated protein kinases (MAPK) includes p38 alpha (SAPK2a, CSBP), p38 beta (SAPK2b), p38 delta (SAPK4), and p38 gamma (SAPK3/ERK6). p38 alpha and p38 beta are widely expressed p38 isoforms that are involved in regulation of cell proliferation, differentiation, development, and response to stress. p38 delta, also known as MAPK13, is a regulator of differentiation-dependent gene expression in keratinocytes, and been as a regulator of surface epithelia differentiation and apoptosis. p38 delta protein is upregulated in Cholangiocarcinoma (CC) relative to hepatocellularcarcinoma (HCC) and to normal biliary tract tissues. p38 delta is important for motility and invasion of CC cells, suggesting that p38 delta may play an important role in CC metastasis. p38 delta is expressed in the epidermis, suggesting a role for p38 delta in regulating differentiation. p38 delta is the major p38 isoform driving suprabasal involucrin gene expression and that p38 delta directly regulates ERK1/2 activity via formation of a p38 delta-ERK1/2 complex. Recent emerging evidence suggests that the p38 stress MAPK pathway may function as a tumor suppressor through regulating Ras-dependent and -independent proliferation, transformation, invasion and cell death by isoform-specific mechanisms. p38 delta has important role in promoting cell proliferation and tumor development in epidermis and may have therapeutic implication for skin cancer.|
|Predicted N Terminal:||Met|
|Form:||Supplied as sterile 50 mM Tris-HCl, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol, pH 7.5.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Bio-activity:||The specific activity was determined to be 55 nmol/min/mg using MBP as substrate.|
|Molecular Mass:||The recombinant human MAPK13/GST chimera consists of 589 amino acids and predicts a molecular mass of 68.4 kDa. It migrates as an approximately 63.9 kDa band in SDS-PAGE under reducing conditions.|
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Purity:||> 90 % as determined by SDS-PAGE|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70 centigrade|
|Storage:||Store it under sterile conditions at -20 centigradeto -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
|Shipping:||Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
|Gene Name:||MAPK13 mitogen-activated protein kinase 13 [ Homo sapiens ]|
|Synonyms:||MAPK13; mitogen-activated protein kinase 13; PRKM13; p38delta; SAPK4; MAPK 13; MAP kinase 13; MAP kinase p38 delta; stress-activated protein kinase 4; mitogen-activated protein kinase p38 delta; MGC99536;|