Active Recombinant Mouse Ephb2 protein(Met1-Lys540), hFc-tagged

• Cat.No.: Ephb2-496M
• Product Overview: Recombinant Mouse EPHB2 (NP_034272.1) (Met1-Lys540) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-hFc
• Protein length: Met1-Lys540
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EFNB2-His at 10 μg/mL (100 μL/well) can bind mouse EPHB2-Fc. The EC50 of mouse EPHB2-Fc is 13.2-30.8ng/mL.
• Molecular Mass: The recombinant mouse EPHB2 comprises 760 amino acids and has a predicted molecular mass of 84.6 kDa. The apparent molecular mass of the protein is approximately 96.6 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ephb2 Eph receptor B2 [ Mus musculus ]
• Official Symbol: Ephb2
• Synonyms: EPHB2; Eph receptor B2; ephrin type-B receptor 2; neural kinase; nuk receptor tyrosine kinase; tyrosine-protein kinase receptor EPH-3; tyrosine-protein kinase receptor SEK-3; Drt; Erk; Nuk; Cek5; Hek5; Qek5; Sek3; ETECK; Prkm5; Tyro5
• Gene ID: 13844
• mRNA Refseq: NM_010142
• Protein Refseq: NP_034272