| Description : |
In molecular biology, acetate kinase (EC 2.7.2.1), which is predominantly found in micro-organisms, facilitates the production of acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. The enzyme is important in the process of glycolysis, enzyme levels being increased in the presence of excess glucose. The growth of a bacterial mutant lacking acetate kinase has been shown to be inhibited by glucose, suggesting that the enzyme is involved in excretion of excess carbohydrate. A related enzyme, butyrate kinase, facilitates the formation of butyryl-CoA by phosphorylating butyrate in the presence of ATP to form butyryl phosphate. |
| Source : |
Bacillus stearothermophilus |
| Species : |
Bacillus stearothermophilus |
| Form : |
Lyophilized |
| Bio-activity : |
more than 1,100 U/mg protein |
| Molecular Mass : |
Molecular weight : ca. 160,000 Subunit molecular weight : ca. 40,000 |
| Unit Definition : |
One unit of activity is defined as the amount of AK that forms 1 μmol of ADP per minute at 30°C. |
| Applications : |
The enzyme is useful for determination of acetate or for ATP regeneration system. |
| Stability : |
No detectable decrease in activity up to 65°C. |
| Storage : |
stable at -20°C for at least one year. |
