"AK" Related Products

Native Bacillus stearothermophilus Acetate Kinase

Cat.No.: AK-14B
Product Overview: Native Acetate Kinase was purified from Bacillus stearothermophilus. EC 2.7.2.1
Description: In molecular biology, acetate kinase (EC 2.7.2.1), which is predominantly found in micro-organisms, facilitates the production of acetyl-CoA by phosphorylating acetate in the presence of ATP and a divalent cation. The enzyme is important in the process of glycolysis, enzyme levels being increased in the presence of excess glucose. The growth of a bacterial mutant lacking acetate kinase has been shown to be inhibited by glucose, suggesting that the enzyme is involved in excretion of excess carbohydrate. A related enzyme, butyrate kinase, facilitates the formation of butyryl-CoA by phosphorylating butyrate in the presence of ATP to form butyryl phosphate.
Source: Bacillus stearothermophilus
Species: Bacillus stearothermophilus
Form: Lyophilized
Bio-activity: more than 1,100 U/mg protein
Molecular Mass: Molecular weight : ca. 160,000 Subunit molecular weight : ca. 40,000
Unit Definition: One unit of activity is defined as the amount of AK that forms 1 μmol of ADP per minute at 30°C.
Applications: The enzyme is useful for determination of acetate or for ATP regeneration system.
Stability: No detectable decrease in activity up to 65°C.
Storage: stable at -20°C for at least one year.

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