Recombinant Human HTRA1, His-tagged

• Cat.No.: HTRA1-47H
• Product Overview: Recombinant human HTRA1 (23-480) protein was secretion-expressed by baculovirus in sf9 insect cells using an N-terminal His-tag.

Specification

• Description: HTRA1 is a serine protease. HTRA1 targets a variety of proteins, including fibronectin (induce synovial cells to up-regulate MMP1 and MMP3 production), proteoglycans (release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space), IGF-binding proteins (regulates the availability of insulin-like growth factors) and TSC2 (leads to the activation of TSC2 downstream targets). HTRA1 relates to age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: His
• Form: 50mM Tris-HCl, pH 8.0, 300mM NaCl, 150mM imidazole and 10% glycerol.
• Molecular Mass: ~58 kDa
• Applications: Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1ug/ul
• Publication:
  HtrA3 as an Early Marker for Preeclampsia: Specific Monoclonal Antibodies and Sensitive High-Throughput Assays for Serum Screening (2012)

Gene Information

• Gene Name: HTRA1 HtrA serine peptidase 1 [ Homo sapiens ]
• Official Symbol: HTRA1
• Synonyms: HTRA1; HtrA serine peptidase 1; protease, serine, 11 (IGF binding) , PRSS11; serine protease HTRA1; HtrA; IGFBP5 protease; IGFBP5-protease; protease, serine, 11 (IGF binding); high-temperature requirement A serine peptidase 1; L56; ARMD7; ORF480; PRSS11
• Gene ID: 5654
• mRNA Refseq: NM_002775
• Protein Refseq: NP_002766
• MIM: 602194
• UniProt ID: Q92743
• Chromosome Location: 10q26.3
• Function: insulin-like growth factor binding; peptidase activity; serine-type endopeptidase activity