"Glutathione" Related Products

Glutathione Agarose High Flow

Cat.no: GS-002A
Product Overview: Glutathione Agarose High Flow is an affinity chromatography medium for easy, one-step purification of glutathione S-transferase (GST) fusion proteins, other glutathione S-transferases and glutathione binding proteins.
Characteristics: Glutathione Agarose HP's performance characteristics include:1. Glutathione coupled to Sepharose High Performance, excellent for high resolution purification of GST-tagged proteins2. Easy one-step purification of GST-tagged proteins and other glutathione S-transferase or glutathionedependent proteins
Size: 25 mL
Matrix: Highly cross-linked Agarose
Average particle size: 90 μm
Ligand: Glutathione
Ligand concentration: 120-320μmol glutathione/ml medium
Coupling chemistry: Epoxy
Dynamic binding capacity: 11mg GST-tagged prtein/ml medium
Recommended flow rate: Sample loading:<100cm>Washing and elution:100-300 cm/h
Recommended column height: 5‐20cm
Chemical stability: stable in 0.1 M acetate pH 4.0, 0.1 M NaOH, 70 % ethanol or 7 M guanidine hydrochloride for two hours at room temperaturenot autoclavable
pH working range: 3-12
Temperature Stability: 2‐40 °C
Shelf life: 5 years

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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